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Environment Friendly Rehydrants for Air-Dried Oral Buccal Smears - A Comparative Study with Conventional Method
Tanvi Handa1, Shally Gupta1, Anubha Gulati1
1Department of Oral Pathology and Microbiology, Dr. Harvansh Singh Judge Institute of Dental Sciences and Hospital, Panjab University, Chandigarh, India.
Journal of Cytology
|January 29, 2024
Summary
This study found aqueous glycerine superior for rehydrating air-dried cytopathology smears compared to alcohol fixation or coconut oil. Coconut oil is also effective for cytoplasmic evaluation in resource-limited settings.
Area of Science:
- Cytopathology
- Histopathology
- Diagnostic Pathology
Background:
- Cytopathology aids disease diagnosis by examining cells.
- Conventional alcohol fixation is problematic in resource-limited settings.
- Air-drying followed by rehydration offers a potential alternative.
Purpose of the Study:
- Assess cytomorphologic changes in air-dried, rehydrated smears.
- Compare rehydration agents (aqueous glycerine, coconut oil) to conventional wet fixation.
- Identify alcohol alternatives for less-equipped areas.
Main Methods:
- Prepared 72 smears from 24 patients (12 healthy, 12 with habits).
- Control group: alcohol fixation (24 smears).
- Test groups: air-dried and rehydrated with aqueous glycerine (24 smears) or coconut oil (24 smears) for 5-10 minutes.
- All smears stained with Papanicolaou stain and scored for cytomorphologic changes.
Main Results:
- Aqueous glycerine demonstrated superior rehydration compared to alcohol-fixed smears.
- Coconut oil showed comparable results to aqueous glycerine for cytoplasmic evaluation.
- Both rehydration methods preserved cytomorphologic details effectively.
Conclusions:
- Aqueous glycerine is a superior rehydrant for air-dried smears in cytopathology.
- Coconut oil is a viable alternative for cytoplasmic evaluation, especially in resource-limited settings.
- These findings support accessible cytopathology techniques in diverse healthcare environments.
