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Micropropagation of Chayote (Sechium edule L.) var. virens levis in RITA®
Lizandro Ramírez-Trejo1, Rosalía Núñez-Pastrana1, Anell Soto-Contreras1
1Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, Amatlán de los Reyes, Veracruz, Mexico.
Methods in Molecular Biology (Clifton, N.J.)
|January 29, 2024
Summary
Chayote (Sechium edule) micropropagation in bioreactors yields more, larger, and vigorous plants compared to traditional methods. This study details a protocol for efficient chayote propagation using RITA® bioreactors for growers.
Area of Science:
- Horticulture
- Plant Biotechnology
- Agricultural Science
Background:
- Chayote (Sechium edule), a member of the nutritious and medicinal Cucurbitaceae family, is gaining international market presence.
- Vigorous and healthy chayote plants are crucial for agricultural producers interested in in vitro propagation.
- Bioreactors offer a high-potential alternative for plant propagation, surpassing traditional semi-solid medium methods.
Purpose of the Study:
- To report a micropropagation protocol for chayote (Sechium edule).
- To optimize chayote plant propagation using RITA® bioreactors.
Main Methods:
- Micropropagation of Sechium edule using RITA® bioreactors.
- Utilizing liquid culture medium within bioreactor systems.
Main Results:
- Bioreactor systems produced larger and more vigorous chayote plant material.
- Significant advantages observed in plant material generation compared to semi-solid medium propagation.
Conclusions:
- RITA® bioreactors provide an effective method for mass propagation of healthy and vigorous chayote plants.
- This protocol supports the commercial cultivation of improved chayote varieties.
