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Multiplication of Cupressus guadalupensis Using the RITA® Temporary Immersion System
Luis Alberto Gómez-Reyes1, Esmeralda Judith Cruz-Gutiérrez2, Lorena Jacqueline Gómez-Godínez1
1Laboratorio Agrícola-Forestal, Centro Nacional de Recursos Genéticos, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP), Tepatitlan de Morelos, Jalisco, Mexico.
Abstract:
The Guadalupe cypress (Cupressus guadalupensis S. Watson) is an endangered species included in the list of the NOM-059-SEMARNAT-2010. The presence of wild goats in the habitat has been the greatest threat to the propagation and survival of this species. Therefore, there is a need to generate propagation protocols that facilitate the regeneration of the species. Plant tissue culture offers various possibilities that can facilitate the regeneration of species under some risk. Temporary immersion systems have proven to be an option with various advantages in plant tissue culture, such as increasing the number of seedlings generated and reducing production times, compared to semisolid media. The objective of this chapter is to describe a protocol to propagate Guadalupe cypress tissues in a RITA® temporary immersion system.
