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Updated: Jul 4, 2025
Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae
Published on: January 29, 2020
A Stereological Approach to Quantify Immunohistochemical Staining in Zebrafish Larvae
Dércia Santos1,2, Sandra M Monteiro1,2, Ana Luzio3,4
1Centre for the Research and Technology of Agro-Environmental and Biological Sciences, CITAB and Inov4Agro, Institute for Innovation, Capacity Building and Sustainability of Agri-food Production, Quinta de Prados, Vila Real, Portugal.
None:
Immunohistochemistry (IHC) is a powerful research tool to localize specific antigens in whole-mount or tissue sections of embryos with labeled antibodies based on antigen-antibody interactions. Stereological methods are nowadays an essential tool to quantify cells or other types of structures in an unbiased and reproducible manner. In this chapter, a general protocol for a stereological estimation of the relative volume density of each structural component (Vv), which can be applied to any organ/structural component, will be described.
