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Detection of dsRNA by Acridine Orange Staining
Tingshuai Ma1, Yu Zhao1, Xiaofei Cheng2
1College of Plant Protection, Northeast Agricultural University, Harbin, Heilongjiang, China.
Methods in Molecular Biology (Clifton, N.J.)
|January 29, 2024
Abstract
None:
Acridine orange is a nucleic acid binding dye that emits green fluorescence when bound to double-stranded DNA or RNA and red fluorescence when bound to single-stranded DNA or RNA under ultraviolet light. This unique characterization allows it to be used for distinguishing or visualization of dsRNA. Here, we present a convenient and efficient protocol for detecting dsRNA in polyacrylamide gels.
