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Updated: Jul 4, 2025
Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro
Published on: March 18, 2014
Mitochondria targeted biomimetic platform for chemo/photodynamic combination therapy against osteosarcoma
Fengtian Zhang1, Jiaoting Chen2, Weihong Luo3
1Department of Orthopaedics, Second Affiliated Hospital of Soochow University Osteoporosis Research Institute of Soochow University, 1055 Sanxiang Road, Suzhou 215000, People's Republic of China; Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases of Ministry of Education, Gannan Medical University, University Park in Rongjiang New District, Ganzhou 341000, People's Republic of China; Department of Orthopedics, First Affiliated Hospital of Gannan Medical University, Jinling East Avenue, Zhanggong District, Ganzhou 341000, People's Republic of China.
Abstract:
Clinical treatment for osteosarcoma (OS) is still lacking effective means, and no significant progress in OS treatment have been made in recent years. Single chemotherapy has serious side effects and can produce drug resistance easily, resulting poor therapeutic effect. As a modern and non-invasive treatment form, photodynamic therapy (PDT) is widely used to treat diverse cancers. Chemotherapy in combination with PDT is a particularly efficient antitumor method that could overcome the defects of monotherapies. Since mitochondria is a key subcellular organelle involved in cell apoptosis regulation, targeting tumor cells mitochondria for drug delivery has become an important entry point for anti-tumor therapy. Herein, we rationally designed a core-shell structured biomimetic nanoplatform, i.e., D@SLNP@OSM-IR780, to achieve tumor homologous targeting and mitochondria targeted drug release for chemotherapy combined with PDT against OS. Upon 808 nm laser irradiation, D@SLNP@OSM-IR780 exhibited excellent photo-cytotoxicity in vitro. The excellent targeting effect of D@SLNP@OSM-IR780 in tumor tissues produced a tumor inhibition rate of 98.9% in vivo. We further indicated that synergistic chemo-photodynamic effect induced by D@SLNP@OSM-IR780 could activate mitochondria-mediated apoptosis pathway, along with host immune response and potential photothermal effect. On the whole, D@SLNP@OSM-IR780 is revealed to be a promising platform for OS targeted combination therapeutics.
Insights
A novel nanoplatform, D@SLNP@OSM-IR780, effectively targets osteosarcoma (OS) cells and mitochondria. This combined chemo-photodynamic therapy achieves significant tumor inhibition and activates apoptosis for improved OS treatment.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Oncology
Background:
- Osteosarcoma (OS) treatment remains challenging due to chemotherapy limitations like severe side effects and drug resistance.
- Photodynamic therapy (PDT) offers a non-invasive approach for cancer treatment, and its combination with chemotherapy shows enhanced efficacy.
- Mitochondria-targeted drug delivery is a key strategy to induce apoptosis in cancer cells.
Purpose of the Study:
- To design and evaluate a core-shell biomimetic nanoplatform (D@SLNP@OSM-IR780) for targeted chemotherapy and PDT in osteosarcoma.
- To investigate the synergistic therapeutic effects of combined chemo-PDT mediated by the nanoplatform.
- To explore the mechanism of action, including mitochondria targeting, apoptosis induction, and potential photothermal effects.
Main Methods:
- Development of a core-shell structured biomimetic nanoplatform (D@SLNP@OSM-IR780) for homologous tumor targeting and mitochondria-specific drug release.
- In vitro evaluation of photo-cytotoxicity upon 808 nm laser irradiation.
- In vivo studies to assess tumor targeting efficiency and therapeutic efficacy, including tumor inhibition rates.
- Analysis of the synergistic chemo-photodynamic effect and its impact on mitochondria-mediated apoptosis pathways and host immune response.
Main Results:
- D@SLNP@OSM-IR780 demonstrated excellent photo-cytotoxicity in vitro.
- The nanoplatform exhibited significant tumor targeting in vivo, achieving a tumor inhibition rate of 98.9%.
- Synergistic chemo-PDT effects were confirmed, activating mitochondria-mediated apoptosis and potentially inducing photothermal effects and host immune responses.
Conclusions:
- The D@SLNP@OSM-IR780 nanoplatform is a promising strategy for targeted combination therapy against osteosarcoma.
- This approach effectively overcomes the limitations of monotherapies by combining chemotherapy and PDT with enhanced tumor targeting and mitochondria-specific drug delivery.
- The developed platform holds potential for advancing osteosarcoma treatment through synergistic therapeutic effects.
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