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FOXO3a functions as a transcriptional and co-transcriptional splicing regulator in vascular endothelial cell lines

Shajidan Abudureyimu1, Chunhui He2, Wei Xie3

  • 1Department of Comprehensive Internal Medicine, The First Affiliated Hospital of Xinjiang Medical University, 830011 Urumqi, Xinjiang, China.

Gene
|January 29, 2024
PubMed

Insights

Forkhead box O3a protein influences vascular endothelial cells by regulating inflammation and lipid metabolism. This study clarifies its role in coronary artery disease pathogenesis.

Area of Science:

  • Molecular Biology
  • Cardiovascular Research
  • Cell Biology

Background:

  • Recent studies link Forkhead box O3a (FOXO3a) protein to coronary artery disease.
  • The precise function of FOXO3a in vascular endothelial cell metabolic regulation and apoptosis remains unclear.

Purpose of the Study:

  • To investigate the role of FOXO3a in regulating target genes within human vascular endothelial cells.
  • To elucidate the impact of FOXO3a overexpression on gene expression and splicing in these cells.

Main Methods:

  • Utilized high-throughput sequencing to analyze gene expression profiles and alternative splicing.
  • Created a human vascular endothelial cell model with FOXO3a overexpression (FOXO3a-OE).
  • Compared gene expression between FOXO3a-OE cells and control cells.

Main Results:

  • Identified 419 differentially expressed genes (DEGs) between FOXO3a-OE and control cells.
  • KEGG pathway analysis revealed upregulated genes enriched in inflammation-related pathways.
  • Downregulated genes were significantly enriched in lipid metabolism-related pathways.

Conclusions:

  • FOXO3a plays a significant role in modulating inflammation and lipid metabolism pathways in vascular endothelial cells.
  • These findings contribute to understanding the molecular mechanisms underlying FOXO3a's involvement in coronary artery disease.
Keywords:
Alternative splicingCoronary heart diseaseFOXO3aTargeted regulation

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