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Related Experiment Video

Updated: Jul 23, 2026

Thin-layer Chromatographic TLC Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Thin-layer Chromatographic TLC Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Published on: March 27, 2014

Mycotoxin detection in selected medicinal plants using chromatographic techniques.

Ihsan Ul Haq¹, Raheela Taj¹, Muhammad Nafees²

¹Institute of Chemical Sciences, University of Peshawar, Peshawar, KPK, Pakistan.

Biomedical Chromatography : BMC

|January 31, 2024

Summary

Medicinal herbs can be contaminated with toxic mycotoxins like aflatoxins and ochratoxin A. Regular monitoring is crucial to ensure the safety of herbal medicines and protect consumers from potential health risks.

Keywords:

HPLC TLC aflatoxin mycotoxin ochratoxin A

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Area of Science:

Food Science
Analytical Chemistry
Pharmacology

Background:

Mycotoxins are toxic compounds produced by fungi that pose significant health risks to humans.
Medicinal herbs and shrubs are increasingly used globally, necessitating an evaluation of their safety regarding mycotoxin contamination.

Purpose of the Study:

To assess the contamination levels of aflatoxins (AFB1, AFB2, AFG1, AFG2) and ochratoxin A (OTA) in selected medicinal herbs and shrubs.
To determine the risk associated with the consumption of herbal decoctions due to mycotoxin presence.

Main Methods:

Analysis of 15 medicinal herb and shrub samples.
Quantification of mycotoxins using thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC).

Main Results:

Four samples showed aflatoxin contamination, with Justicia adhathoda exhibiting the highest levels.
Two samples were contaminated with ochratoxin A, with Dodonia viscosa showing the highest levels.
OTA levels were generally low and below permissible limits, but aflatoxin levels in some samples were concerning.

Conclusions:

Consumption of herbal decoctions carries a potential risk due to mycotoxin contamination.
Regular monitoring and management of mycotoxins in herbal medicines are essential for consumer safety.