Protocol for building a user-friendly temperature control system to support microscopes, microfluidic chambers, and custom incubators
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Published on: March 19, 2019
Ziteng Liu1, Thao Thi Thu Nguyen2, Fangyuan Ding3
1Department of Electrical Engineering and Computer Science, University of California Irvine, Irvine, CA 92697, USA.
Abstract:
Biological experiments require precise temperature control, necessitating an integrated adjustable temperature system for instruments such as microscopes, microfluidic chambers, or custom incubators. We present a protocol for building a user-friendly temperature control system suitable for both in vitro and in vivo assays. We describe steps for preparing materials, assembling the printed circuit board and enclosure, and fine-tuning the heating algorithm for accuracy. This system can maintain a stable temperature of up to 60°C with stabilities under 0.06°C.

