Related Experiment Video
Updated: Jul 4, 2025

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo
Published on: February 6, 2015
Vitamin B12 Protects against Genotoxicity Induced by Cisplatin
Wejdan M Al-Bataineh1, Karem H Alzoubi2,3, Omar F Khabour4
1Department of Applied Biological Sciences, Faculty of Science and Art, Jordan University of Science and Technology, Irbid, Jordan.
Background:
Cisplatin is an effective synthetic chemotherapeutic drug used for cancer treatment. Vitamin B12 has been shown to possess anti-genotoxic activity. This study aimed to investigate the effect of vitamin B12 on chromosomal damage induced by cisplatin.
Methods:
The level of sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs) were measured in cultured human blood lymphocytes treated with cisplatin and/or vitamin B12.
Results:
The results showed a significantly elevated frequency of CAs and SCEs of cisplatin-treated cultures compared to the control (P < 0.05). The CAs and SCEs induced by cisplatin were significantly lowered by pretreatment of cell cultures with vitamin B12. In addition, cisplatin caused a slight reduction in the mitotic index (MI), while vitamin B12 did not modulate the effect of cisplatin on MI.
Conclusion:
Vitamin B12 can protect human lymphocytes against genotoxicity associated with cisplatin.
Related Concept Videos
DNA Damage can Stall the Cell Cycle
Mutagenicity and Carcinogenicity
Abnormal Proliferation
Cancer Prevention
Some...

