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Urine LMs quantitative analysis strategy development and LMs CWP biomarkers discovery
Yicong Geng1, Chunmin Zhang2, Ping He1
1State Key Laboratory for Fine Exploration and Intelligent Development of Coal Resources, China University of Mining and Technology-Beijing, Ding11 Xueyuan Road, Beijing 100083, China; School of Chemical and Environmental Engineering, China University of Mining and Technology-Beijing, Ding11 Xueyuan Road, Beijing 100083, China.
Early diagnosis of coal workers' pneumoconiosis (CWP) is crucial. Urinary lipid mediators like 9-OxoODE and 13-OxoODE show promise as biomarkers for detecting CWP, aiding in timely intervention.
Area of Science:
- Occupational Medicine
- Biochemistry
- Analytical Chemistry
Background:
- Coal workers' pneumoconiosis (CWP) is a prevalent occupational disease with no effective treatments.
- Early diagnosis of CWP is vital for reducing mortality rates.
- Lipid mediators (LMs) are key regulators of inflammation and potential biomarkers for inflammatory diseases like CWP.
Purpose of the Study:
- To identify urinary lipid mediators as potential biomarkers for the early diagnosis of CWP.
- To analyze the metabolic network of LMs in CWP patients and control groups.
- To investigate the association between enzyme pathways and CWP.
Main Methods:
- Utilized Ultra-High-Performance Liquid Chromatography-Multiple Reaction Monitoring (UHPLC-MRM) to detect urinary LMs.
- Performed comprehensive metabolic network analysis of LMs in CWP and control (CT) groups.
- Employed receiver operating characteristic (ROC) analysis and logistic regression for biomarker validation.
Main Results:
- Urinary levels of 13-OxoODE, 9-OxoODE, and 9,10-EpOME were significantly elevated in the CWP group (P < 0.05).
- ROC analysis demonstrated good diagnostic potential for these LMs, with areas under the curve ranging from 68.8% to 88.8%.
- Significant abnormalities in lipoxygenase (LOX) and cytochrome P450 (CYP450) enzyme pathways were observed in CWP, with CYP450 changes potentially linked to polycyclic aromatic hydrocarbons (PAHs).
Conclusions:
- 13-OxoODE, 9-OxoODE, and 9,10-EpOME are identified as potential biomarkers for the early diagnosis of CWP.
- Abnormalities in LOX and CYP450 enzyme pathways are associated with CWP.
- These findings contribute to the development of strategies for early CWP diagnosis and treatment.
