Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

51.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
51.0K
DNA-only Transposons02:57

DNA-only Transposons

14.5K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
14.5K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

15.5K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.5K
Homologous Recombination02:31

Homologous Recombination

50.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-read Sequencing: from Complete Molecules to Context-resolved Biology.

Genomics, proteomics & bioinformatics·2026
Same author

Association between weekend catch-up sleep and metabolic syndrome: A cross-sectional study.

Medicine·2026
Same author

Food-derived peptides for senile sarcopenia: mechanisms of action, structural characteristics, and <i>in vivo</i> delivery challenges.

Food & function·2026
Same author

Risk factors associated with growth pain disorder in children: a systematic review and meta-analysis.

Frontiers in pediatrics·2026
Same author

Unlocking Superconductivity in NdFeAsO via Uranium-Mediated Electron Doping.

Inorganic chemistry·2026
Same author

Cosmetic exposure and hepatic steatosis: Investigating the role of metals and adipokines.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2026
Same journal

Unifying Repbase and Dfam: a new open foundation for transposable element research.

Mobile DNA·2026
Same journal

Dynamic transposable element expression during Hydra head regeneration.

Mobile DNA·2026
Same journal

Glomhopper-a subfamily of DUF3504-encoding CryptonA elements in Glomeromycota.

Mobile DNA·2026
Same journal

Stones in genomic storms: transposable element-driven variation in Prunus fruit trees.

Mobile DNA·2026
Same journal

Transposable elements in hematopoietic stem cells upon aging and myeloid malignancies.

Mobile DNA·2026
Same journal

Howler monkey Platy-1 and Alu SINEs: a resource for Alouatta genomics.

Mobile DNA·2026
See all related articles

Related Experiment Video

Updated: Jul 4, 2025

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.4K

CRISPR-TE: a web-based tool to generate single guide RNAs targeting transposable elements.

Yixin Guo1, Ziwei Xue1,2, Meiting Gong1

  • 1Department of Orthopedic Surgery of the Second Affiliated Hospital, and Centre of Biomedical Systems and Informatics of Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, Zhejiang University, Zhejiang, Hangzhou, 310003, China.

Mobile DNA
|February 2, 2024
PubMed
Summary
This summary is machine-generated.

CRISPR-TE is a new tool for designing single guide RNAs (sgRNAs) to efficiently target transposable elements (TEs). This web application aids researchers in manipulating TEs for functional studies in the human and mouse genomes.

More Related Videos

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

22.0K
CRISPR Guide RNA Cloning for Mammalian Systems
06:48

CRISPR Guide RNA Cloning for Mammalian Systems

Published on: October 2, 2018

70.6K

Related Experiment Videos

Last Updated: Jul 4, 2025

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.4K
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

22.0K
CRISPR Guide RNA Cloning for Mammalian Systems
06:48

CRISPR Guide RNA Cloning for Mammalian Systems

Published on: October 2, 2018

70.6K

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • CRISPR/Cas systems are powerful genome engineering tools.
  • Transposable elements (TEs) play crucial roles, necessitating their manipulation for functional studies.
  • Designing specific and efficient sgRNAs for repetitive TEs is challenging.

Purpose of the Study:

  • To develop an optimized sgRNA designer tool for transposable element (TE) manipulation.
  • To provide a comprehensive solution for efficient TE targeting at single copy and subfamily levels.
  • To facilitate research on TE functions using CRISPR/Cas technology.

Main Methods:

  • Development of a web-based application, CRISPR-TE, with a graphical user interface.
  • Tailoring the tool for human and mouse genomes.
  • Identification of potential sgRNAs specific for TEs.

Main Results:

  • CRISPR-TE successfully identifies sgRNAs for TE targeting.
  • The tool enables efficient targeting of TEs at both single copy and subfamily levels.
  • sgRNAs targeting TEs are more effective for evolutionarily young TEs with conserved sequences.

Conclusions:

  • CRISPR-TE provides a versatile framework for designing sgRNAs for TE targeting.
  • The application is publicly accessible as an online web service.
  • The source code for CRISPR-TE is available for further development and use.