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Related Experiment Video

Updated: Jul 4, 2025

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter
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Hydroxyapatite-Gold Modified Screen-Printed Carbon Electrode for Selective SARS-CoV-2 Antibody Immunosensor.

Ratu Shifa Syafira1, Melania Janisha Devi1, Shabarni Gaffar1

  • 1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Jl. Raya Bandung-Sumedang Km 21, Jatinangor, Sumedang, West Java 45363, Indonesia.

ACS Applied Bio Materials
|February 2, 2024
PubMed
Summary

A novel electrochemical immunosensor rapidly detects SARS-CoV-2 antibodies. This screen-printed carbon electrode immunosensor utilizes a hydroxyapatite-gold nanocomposite and the RBD Spike protein for high sensitivity and stability in detecting COVID-19 antibodies.

Keywords:
Anti-RBD SpikeElectrochemicalHA-AuRBD SpikeSARS-CoV-2SPCEimmunosensor

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • The global spread of SARS-CoV-2 necessitates rapid and accurate diagnostic tools.
  • Electrochemical immunosensors offer a promising alternative for swift viral detection.

Purpose of the Study:

  • To develop a highly sensitive and stable electrochemical immunosensor for detecting SARS-CoV-2 antibodies.
  • To optimize the synthesis of a hydroxyapatite-gold nanocomposite for enhanced electrode performance.

Main Methods:

  • Fabrication of a screen-printed carbon electrode immunosensor (SPCE/HA-Au) with immobilized RBD Spike protein.
  • Optimization of HA-Au composite synthesis using the Box-Behnken method.
  • Characterization via UV-vis, TEM-EDX, XRD, differential pulse voltammetry, and electrochemical impedance spectroscopy.

Main Results:

  • The developed immunosensor achieved a low detection limit of 0.0561 pg mL⁻¹ for IgG antibodies.
  • Demonstrated excellent selectivity (103-122%) and stability up to 7 weeks.
  • Validated with real human serum samples, showing satisfactory agreement with CMIA results.

Conclusions:

  • The SPCE/HA-Au based electrochemical immunosensor provides a rapid, accurate, and stable platform for SARS-CoV-2 antibody detection.
  • This technology holds potential for effective COVID-19 monitoring and diagnosis.