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Bacterial Laccase: Recent Advances in Production, Engineering Strategies, and Diagnostic Applications
Fina Wulandari Mardiyono1,2, Bunga Septiyadi Aulia Putri1,2, Asrul Muhammad Fuad3
1School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, West Java, Indonesia.
Biotechnology and Bioengineering
|June 18, 2026
Summary
Bacterial laccases, versatile enzymes, show promise in diagnostics and industry. Advances in production and engineering enhance their stability and catalytic properties for practical applications.
Area of Science:
- Biochemistry
- Enzymology
- Synthetic Biology
Background:
- Bacterial laccases are multicopper oxidases with high stability.
- They catalyze oxidation reactions with oxygen as the sole byproduct.
- Recombinant production is feasible in various microbial hosts.
Purpose of the Study:
- Review recent advances in bacterial laccase research over the past decade.
- Focus on production technologies, protein engineering, and diagnostic applications.
- Assess the transition of bacterial laccases into practical biocatalysts.
Main Methods:
- Review of over 120 peer-reviewed papers.
- Analysis of synthetic biology for heterologous expression.
- Evaluation of protein engineering strategies (directed evolution, rational design).
- Assessment of computational modeling and post-translational modifications.
- Examination of diagnostic applications in biosensing and point-of-care diagnostics.
Main Results:
- Synthetic biology has enabled industrial-scale production in hosts like Escherichia coli and Bacillus subtilis.
- Protein engineering has significantly enhanced laccase catalytic properties.
- Bacterial laccases show potential as peroxidase alternatives in clinical analyte detection (dopamine, uric acid, glucose).
- These enzymes are transitioning to practical use in environmental monitoring, industrial processing, and medical diagnostics.
Conclusions:
- Bacterial laccases are evolving into valuable biocatalysts with diverse applications.
- Further research is needed to overcome remaining challenges and realize their full potential.
- Future directions include optimizing production and engineering for specific industrial and diagnostic needs.
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