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Updated: Jul 26, 2026
Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit
Published on: August 2, 2017
Protocol for induction of heterosynaptic long-term potentiation in the mouse hippocampus via dual-opsin stimulation
Fengwen Huang1, Stephen Temitayo Bello1, Abdul Baset1
1Department of Neuroscience, City University of Hong Kong, Kowloon Tong, Hong Kong 0000, China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science & Innovation, Chinese Academy of Sciences, Hong Kong 0000, China.
Abstract:
Cholecystokinin (CCK) is the most abundant neuropeptide that broadly regulates the physiological status of animals. Here, we present a two-color laser theta burst stimulation (L-TBS) protocol for simultaneous activation of Schaffer collateral and perforant pathway in the hippocampus of CCK Cre mice. We describe steps for heterosynaptic long-term potentiation induction by L-TBS. This technique allows for the examination of the neurotransmitter roles in synaptic modulation and facilitates the exploration of pathological mechanisms in genetic models of brain disorders in mice. For complete details on the use and execution of this protocol, please refer to Su et al.1.
