Related Experiment Video

Updated: Aug 18, 2026

Generation of Fluorescent Protein Fusions in Candida Species
09:27

Generation of Fluorescent Protein Fusions in Candida Species

Published on: March 4, 2017

An inducible proline transport system in Candida albicans

A Jayakumar, M Singh, R Prasad

    Biochimica Et Biophysica Acta
    |September 4, 1979
    PubMed

    More Related Videos

    Bio-energetics Investigation of Candida albicans Using Real-time Extracellular Flux Analysis08:48

    Bio-energetics Investigation of Candida albicans Using Real-time Extracellular Flux Analysis

    Published on: March 19, 2019

    Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis09:44

    Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

    Published on: June 13, 2021

    Related Concept Videos

    ABC Transporters: Importer01:27

    ABC Transporters: Importer

    ATP-binding cassette or ABC transporters are a class of ATP-driven pumps that hydrolyze ATP to move solutes across the membrane. They can be grouped into importers and exporters. While exporters are present in all domains of life, importers exist only in bacteria and some plants.
    In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
    Protein Transport to the Stroma01:24

    Protein Transport to the Stroma

    Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function.
    Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
    Protein Transport to the Outer Chloroplast Membrane01:11

    Protein Transport to the Outer Chloroplast Membrane

    Chloroplast outer membrane proteins encoded by the nucleus are synthesized in the cytosol. Soon after synthesis, they bind cytosolic factors such as 14-3-3 protein and the Hsp70 chaperones that keep these precursors in an unfolded state until their translocation.
    Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
    Protein Transport to the Thylakoids01:22

    Protein Transport to the Thylakoids

    Thylakoids are membrane-bound sac-like structures within the chloroplast that serve as sites for photosynthesis. Thylakoid lumen contains many electron transport proteins and is enclosed by a thylakoid membrane rich in the light-harvesting complex. Proteins targeted to the thylakoids are transported as precursors and are sorted by the general TOC/TIC import pathway. Once the precursor reaches the stroma, stromal processing peptidases remove their transit signal and expose thylakoid signal...
    Protein Transport to the Inner Chloroplast Membrane01:18

    Protein Transport to the Inner Chloroplast Membrane

    Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
    Bacterial Translocation and Protein Secretion01:26

    Bacterial Translocation and Protein Secretion

    Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...