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Paneth cell ontogeny in term and preterm ovine models.
Geoanna M Bautista1, Anjali J Cera1, Rebecca J Schoenauer2
1Department of Pediatrics, University of California, Davis, Sacramento, CA, United States.
Frontiers in Veterinary Science
|February 6, 2024
Summary
Paneth cells are concentrated in the ileum of fetal and newborn lambs, with density decreasing due to perinatal asphyxia. This highlights the ovine model
Area of Science:
- Gastroenterology
- Developmental Biology
- Immunology
Background:
- Paneth cells are crucial for intestinal health, regulating the microbiome and immunity.
- Understanding Paneth cell biology in immature mammals, like sheep, is limited.
- Previous studies focused on adult sheep, leaving a gap in fetal and neonatal knowledge.
Purpose of the Study:
- To investigate the ontogeny, quantification, and localization of Paneth cells in fetal and newborn lambs.
- To compare Paneth cell distribution in ovine fetuses and neonates with human patterns.
- To assess the impact of perinatal transient asphyxia on Paneth cell development.
Main Methods:
- Intestinal samples collected from preterm (126-127 days gestation) and term (140-141 days gestation) lambs.
- Paneth cells quantified per crypt (at least 100 crypts/animal).
- Immunohistochemistry used for Paneth cell confirmation.
Main Results:
- Paneth cells showed significantly higher density in the ileum compared to the jejunum.
- Paneth cells were absent in the colon.
- Perinatal transient asphyxia led to an acute decrease in Paneth cell numbers.
Conclusions:
- Ovine Paneth cell distribution at birth mirrors human patterns, with highest concentrations in the ileum.
- Paneth cell density is gestation-dependent in sheep.
- The ovine model is suitable for studying fetal and neonatal Paneth cell biology, especially concerning asphyxia impacts.
