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Updated: Jul 4, 2025

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans
Published on: February 23, 2019
Evaluation of Oxygen Consumption Rates In Situ
Anqi Li1,2, Yuan Qin3, Ying Zhang2
1Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China.
Abstract:
An analysis of the mitochondrial respiration function represented by the oxygen consumption rate is necessary to assess mitochondrial bioenergetics and redox function. This protocol describes two alternative techniques to evaluate mitochondrial respiration function in situ: (1) measure oxygen consumption rates via an electrode; (2) measure oxygen consumption rates via a seahorse instrument. These in situ approaches provide more physiological access to mitochondria to evaluate mitochondrial respiration function in a relatively integrated cellular system.
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