Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renal Clearance01:23

Renal Clearance

939
The glomerular filtration rate (GFR) is a critical marker of kidney function, reflecting the efficiency of filtration by the glomeruli. Renal clearance of specific substances, such as inulin or creatinine, is commonly used to measure GFR.
Renal clearance refers to the volume of plasma cleared of a specific substance, such as creatinine, per unit of time. To measure clearance, urine samples are collected over a 24-hour period during each bladder voiding, followed by a single blood sample at the...
939
Factors Affecting Renal Clearance: Renal Impairment01:17

Factors Affecting Renal Clearance: Renal Impairment

95
Renal dysfunction significantly impairs the renal clearance of drugs, leading to potential complications in drug therapy. Renal failure, which can be caused by various factors, poses a significant challenge in the elimination of drugs from the body.
One condition associated with renal failure is uremia. Uremia is characterized by impaired glomerular filtration and fluid accumulation in the body. This condition hinders the renal clearance of drugs, resulting in drug accumulation and potential...
95
Physiology of Urine Formation01:24

Physiology of Urine Formation

4.0K
Urine formation is an essential function of the human body. It plays a critical role in maintaining homeostasis by regulating the volume and composition of body fluids. The kidneys, the primary organs involved in this process, filter blood to remove waste products and excess substances, ultimately producing urine.
Glomerular Filtration
The first stage in urine formation is glomerular filtration. Each kidney contains approximately 1 million nephrons, the functional units of filtration, with a...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association of Markers of Kidney Tubular Secretion and Risk of Acute Kidney Injury after Acute Heart Failure.

Kidney360·2026
Same author

Markers of kidney tubule dysfunction and injury and long-term risk of acute kidney injury following coronary artery bypass graft surgery.

PloS one·2026
Same author

Measuring urine albumin and total protein.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association·2026
Same author

Commutability assessment of NIST SRM 3666 albumin and creatinine in frozen human urine among routine clinical laboratory measurement procedures.

Clinica chimica acta; international journal of clinical chemistry·2025
Same author

Quantitative Analysis of a Novel Metabolite Panel to Estimate GFR (Panel eGFR) in Serum and Plasma Using LC-MS/MS.

Clinical chemistry·2025
Same author

Kidney Tubule Secretion Can Discriminate the Cause of Acute Kidney Injury in Cirrhosis.

Clinical journal of the American Society of Nephrology : CJASN·2025

Related Experiment Video

Updated: Jul 4, 2025

Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

17.7K

Urine Albumin Measurements in Clinical Diagnostics.

Jesse C Seegmiller1, Lorin M Bachmann2

  • 1Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN, United States.

Clinical Chemistry
|February 7, 2024
PubMed
Summary

Urine albumin measurement is key for chronic kidney disease (CKD) monitoring. Significant variations in measurement procedures can impact CKD diagnosis and risk classification, highlighting the need for standardization.

More Related Videos

Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70
09:16

Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70

Published on: August 9, 2019

6.9K
Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
10:05

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins

Published on: August 7, 2014

13.9K

Related Experiment Videos

Last Updated: Jul 4, 2025

Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

17.7K
Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70
09:16

Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70

Published on: August 9, 2019

6.9K
Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
10:05

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins

Published on: August 7, 2014

13.9K

Area of Science:

  • Clinical Chemistry
  • Nephrology
  • Biomarker Measurement

Background:

  • Urine albumin measurement is critical for diagnosing, classifying risk, and monitoring chronic kidney disease (CKD).
  • Clinical guidelines use urine albumin-to-creatinine ratio (ACR) cutoffs of 30 and 300 mg/g (3 and 30 mg/mmol).
  • Variations exceeding 40% among manufacturers' measurement procedures can lead to inconsistent CKD diagnosis and risk assessment.

Approach:

  • This review covers urine albumin pathophysiology, CKD guideline recommendations, and best practices for specimen collection, storage, and ACR reporting.
  • It examines recent advancements in measurement techniques and the development of reference systems for standardizing urine albumin measurements.
  • The review discusses the use of immunoturbidimetric and immunonephelometric techniques and their associated variability.

Key Points:

  • Urine albumin results should be reported as ACR using quantitative methods.
  • Confirming random urine collections with first morning void collections improves diagnostic accuracy.
  • Significant variability in measurement procedures can affect CKD classification and risk assessment.

Conclusions:

  • Standardization of urine albumin measurements is essential for accurate CKD diagnosis and risk stratification.
  • Developing reference systems, including mass spectrometry methods and reference materials, is crucial for achieving standardization.
  • Collaborative efforts are needed to ensure consistent and reliable urine albumin testing across different laboratories.