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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jul 4, 2025

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

Correction: Multiple imputation and direct estimation for qPCR data with non-detects

Valeriia Sherina¹, Helene R McMurray^2,3, Winslow Powers⁴

¹Department of Biostatistics and Computational Biology, University of Rochester Medical Center, 265 Crittenden Blvd., Rochester, NY, 14642, USA.

BMC Bioinformatics

|February 7, 2024

Summary

No abstract available in PubMed .

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