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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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P-TDHM: Open-source portable telecentric digital holographic microscope.

Lei Jin1,2, Ziyang Yu1,2, Aaron Au1,2

  • 1Institute of Biomedical Engineering, 164 College St, University of Toronto, Toronto, ON M5S 3G9, Canada.

Hardwarex
|February 8, 2024
PubMed
Summary

We developed a low-cost, portable telecentric digital holographic microscope using readily available parts. This accessible microscopy tool achieves high-resolution imaging for diverse biological samples in various settings.

Keywords:
Holographic microscopyPortable microscopePortable telecentric digital holographic microscopyQuantitative phase imaging

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Area of Science:

  • Biomedical Engineering
  • Optical Microscopy
  • Holography

Background:

  • Digital holographic microscopy (DHM) offers advanced imaging capabilities.
  • Traditional DHM systems can be expensive and complex.
  • There is a need for accessible microscopy solutions in resource-limited environments.

Purpose of the Study:

  • To design and evaluate a low-cost, portable telecentric digital holographic microscope (P-TDHM).
  • To demonstrate the system's performance using various biological and nano-scale samples.
  • To make advanced holographic microscopy more accessible.

Main Methods:

  • Utilized off-the-shelf components for hardware construction.
  • Developed integrated software for system control and image acquisition.
  • Performed imaging experiments with nano-printed targets, HeLa cells, HEK293 cells, and Dolichospermum.
  • Operated the system in both in-line and off-axis holographic modes.

Main Results:

  • Acquired high-quality quantitative phase images.
  • Achieved nanometer axial and sub-micron lateral resolution.
  • Demonstrated successful imaging of diverse samples, including live cells.
  • Confirmed the system's small form factor and portability.

Conclusions:

  • The P-TDHM is a cost-effective and portable solution for advanced microscopy.
  • The system provides high-resolution imaging suitable for various applications.
  • This design significantly enhances the accessibility of telecentric digital holographic microscopy.