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CDX2 dose-dependently influences the gene regulatory network underlying human extraembryonic mesoderm development
Emily A Bulger1,2, Todd C McDevitt1,3, Benoit G Bruneau1,4,5,6,7
1Gladstone Institutes, San Francisco, CA.
Biorxiv : the Preprint Server for Biology
|February 8, 2024
Summary
CDX2 gene dosage critically impacts early embryonic development, particularly extraembryonic mesoderm. This study reveals CDX2 dose-dependently regulates genes for cytoskeletal integrity and signaling pathways, crucial for allantois development.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Genetics
Background:
- Proper gene dosage is essential for early embryonic development and extraembryonic tissues.
- Loss of CDX2 in vivo impairs allantois development, a structure vital for nutrient and waste exchange.
Conclusions:
- CDX2 dosage critically influences gene expression in extraembryonic mesoderm, impacting cytoskeletal and signaling pathways.
- Chromatin accessibility alone is not sufficient to rescue gene expression when CDX2 dosage is reduced.
- Commonly misregulated genes in CDX2 and TBXT loss-of-function models may explain defects in vascular development and allantoic elongation.
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