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Updated: Jul 4, 2025

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
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Mechanism of Erianin anti-triple negative breast cancer based on transcriptomics methods and network pharmacology

Ming Li1, Yuan Zhao2, Huimin Li1

  • 1Laboratory of Molecular Genetics of Aging and Tumour, Medical School, Kunming University of Science and Technology, Chenggong Campus, Kunming, Yunnan 650500, China.

Aging
|February 8, 2024
PubMed

Insights

Erianin effectively inhibits triple negative breast cancer (TNBC) proliferation and tumor growth by downregulating the PI3K/AKT signaling pathway, offering a promising new treatment avenue.

Area of Science:

  • Oncology
  • Pharmacology
  • Bioinformatics

Background:

  • Triple negative breast cancer (TNBC) is an aggressive cancer subtype lacking targeted therapies.
  • Erianin exhibits potential antitumor activity, but its mechanism in TNBC is not fully understood.

Purpose of the Study:

  • To elucidate the mechanism of action of Erianin in TNBC using transcriptomics and network pharmacology.
  • To identify key molecular targets and pathways affected by Erianin in TNBC.

Main Methods:

  • Transcriptomics and network pharmacology were employed to identify Erianin's targets.
  • In vitro TNBC cell lines and in vivo xenograft models were used for bioactivity evaluation.
  • Molecular docking and Western blot analyses were performed to validate targets and pathways.

Main Results:

  • Erianin significantly inhibited TNBC cell proliferation and tumor growth.
  • Fifty-one mutual targets were identified, with eight hub targets highlighted, including PPARA and ROCK2.
  • Erianin was found to suppress the PI3K/Akt signaling pathway, confirmed by Western blot and SC79 co-incubation experiments.

Conclusions:

  • Erianin demonstrates therapeutic potential for TNBC by inhibiting proliferation and tumor growth.
  • The PI3K/AKT signaling pathway is a key mediator of Erianin's anti-TNBC effects.
  • Erianin represents a promising candidate for novel TNBC drug development.
Keywords:
Erianinmolecular mechanismnetwork pharmacologytranscriptomicstriple-negative breast cancer

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