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Related Experiment Videos

Free l-carnitine in human semen.

G Abbaticchio, V A Giagulli, M Defini

    Archives of Andrology
    |January 1, 1985
    PubMed
    Summary

    This study developed a spectrophotometric assay for seminal carnitine (SC). SC levels were inversely correlated with semen volume and may serve as an index of androgenization.

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    Area of Science:

    • Reproductive Biology
    • Biochemistry
    • Analytical Chemistry

    Background:

    • Seminal carnitine (SC) plays a role in sperm maturation and function.
    • Accurate measurement of SC is crucial for understanding male reproductive health.

    Purpose of the Study:

    • To develop and validate a spectrophotometric assay for quantifying SC in seminal plasma.
    • To investigate the relationship between SC levels and semen parameters.
    • To explore SC levels in various male infertility conditions.

    Main Methods:

    • Spectrophotometry at 412 nm was used to measure SC.
    • Assay validation included linearity, sensitivity, and repeatability assessments.
    • SC levels were correlated with semen volume and analyzed in normozoospermic and oligozoospermic subjects.

    Main Results:

    • The assay demonstrated good linearity (r=0.998) and sensitivity (0.70 microgram/ml) with minimal interassay variation (8%).
    • SC levels were inversely correlated with semen volume (r=0.49).
    • No significant difference in ejaculated SC was found between normozoospermic and oligozoospermic groups.
    • Absent or very low SC levels were observed in cases of agenesis, monorchidism, and varicocele.

    Conclusions:

    • A reliable spectrophotometric method for SC quantification was established.
    • Seminal carnitine levels are linked to semen volume and may indicate androgenization.
    • The epididymis is confirmed as a primary site of SC production.
    • SC levels may serve as a biomarker for certain male reproductive conditions.

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