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A stable and sensitive 2D SERS sensor for bioanalytical applications.

Marianna Pannico1, Pellegrino Musto1

  • 1National Research Council of Italy, Institute for Polymers, Composites and Biomaterials, 80078 Pozzuoli (NA), Italy.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|February 8, 2024
PubMed
Summary

We developed a 2D-Surface-Enhanced Raman Spectroscopy (SERS) sensor using gold nanoparticles for sensitive detection. This sensor shows potential for accurate Therapeutic Drug Monitoring (TDM) in the biomedical field.

Keywords:
6-mercaptopurineAFMRaman imagingSERSTherapeutic Drug Monitoring

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Area of Science:

  • Nanotechnology
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Surface-Enhanced Raman Spectroscopy (SERS) offers high sensitivity for molecular detection.
  • Developing stable and uniform SERS substrates is crucial for reliable analytical measurements.
  • Therapeutic Drug Monitoring (TDM) requires sensitive and accurate methods for drug quantification.

Purpose of the Study:

  • To create a 2D-SERS sensor using gold nanoparticles (AuNPs) on a functionalized metal surface.
  • To evaluate the sensor's performance for detecting 6-mercaptopurine (6-MP) for TDM.
  • To demonstrate the potential of the 2D-SERS sensor for hypersensitive analytical applications.

Main Methods:

  • Fabrication of a 2D-SERS sensor via deposition of AuNPs onto a functionalized metal surface.
  • Morphological characterization using Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM).
  • Functionalization with p-mercaptoaniline (pMA) and Raman spectroscopy for signal enhancement and homogeneity assessment.

Main Results:

  • SEM and AFM confirmed uniform and stable distribution of AuNPs.
  • pMA functionalization led to significant Raman signal enhancement and homogeneous SERS activity.
  • The sensor demonstrated a linear correlation between 6-MP concentration (5–20 μM) and SERS intensity.

Conclusions:

  • The developed 2D-SERS sensor exhibits excellent sensitivity and homogeneity.
  • The sensor is suitable for Therapeutic Drug Monitoring (TDM) applications, specifically for 6-mercaptopurine.
  • This technology holds promise for advancing hypersensitive analytical measurements in the biomedical field.