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Published on: January 18, 2016
Identification and functional characterization of two antenna-specifc odorant-binding proteins in Plutella xylostella
Buguo Wang1, Yongjie Zhang1, Chenyang Zhang1
1School of Plant Protection, Anhui Agricultural University, Hefei 230036, China; Anhui Province Engineering Laboratory for Green Pesticide Development and Application, Hefei 230036, China; Anhui Province Key Laboratory of Crop Integrated Pest Management, Hefei 230036, China.
Abstract:
Plutella xylostella is an important cruciferous crop pest with a serious resistance to multiple insecticides, a novel natural compound, 2,3-dimethyl-6-(1-hydroxy)-pyrazine were isolated, that showed significant repellent activity against P. xylostella with olfactory system as a potential target. Eight odorant-binding proteins (OBPs) were determined as candidate target genes using RT-qPCR (Quantitative reverse transcription PCR), most of them were clustered with OBPs from Spodoptera frugiperda. Fluorescence competitive binding assays showed that PxylPBP2 (Pheromone binding protein) and PxylOBP3 had Ki values of 7.13 ± 0.41 μM and 9.56 ± 0.35 μM, indicating a high binding affinity to the pyrazine. Moreover, the binding style between these two OBPs and the pyrazine was determined as a hydrophobic interaction by using molecular docking. The binding between PxylPBP2 and the pyrazine was found to be more stable, and the carbon atoms of C-2 and C-3 in this pyrazine showed potential optimization characteristics. Both PxylPBP2 and PxylOBP3 were highly expressed in the antennae of both sexes. These results can be used to design and develop novel green pesticides with the pyrazine as the active or lead compound to reduce the utilization of chemical pesticides and postpone development of resistance.
