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SDS-PAGE

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...

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Related Experiment Video

Updated: Apr 30, 2026

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

Published on: October 28, 2016

An efficient method for detecting membrane protein oligomerization and complex using 05SAR-PAGE.

Liqun Huang¹, Qiong Tong², Lang Chen²

¹Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, P. R. China.

Electrophoresis

|February 9, 2024

Summary

This study introduces 0.05% sarkosyl-polyacrylamide gel electrophoresis (05SAR-PAGE) as a sensitive method for identifying membrane protein oligomerization. This technique simplifies the analysis of protein complexes and weak interactions.

Keywords:

05SAR‐PAGE complex membrane protein oligomer weak interaction

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Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

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Related Experiment Videos

Last Updated: Apr 30, 2026

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

Published on: October 28, 2016

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Published on: May 5, 2017

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

Area of Science:

Biochemistry
Molecular Biology
Proteomics

Background:

Protein oligomerization is crucial for biological function, especially in membrane proteins.
Characterizing protein oligomerization states is often complex and labor-intensive.

Purpose of the Study:

To develop a sensitive, efficient, and practical method for identifying membrane protein oligomerization states.
To analyze protein complexes and weak interactions in cell lysates.

Main Methods:

Utilized 0.05% sarkosyl-polyacrylamide gel electrophoresis (05SAR-PAGE) for high-sensitivity oligomer state identification.
Employed two-dimensional electrophoresis (05SAR/SDS-PAGE) coupled with western blotting and LC-MS/MS for complex analysis (CpxA/OmpA).

Main Results:

Successfully identified oligomer states of membrane proteins CpxA, EnvZ, and Ma-Mscl using 05SAR-PAGE.
Demonstrated the efficacy of 05SAR-PAGE in analyzing the CpxA/OmpA complex in cell lysate.

Conclusions:

05SAR-PAGE is an efficient, economical, and practical gel-based method for membrane protein oligomerization studies.
This technique is suitable for analyzing weak protein interactions and protein complexes.