Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Subplasmalemmal calcium-binding microregions in Acanthamoeba.

A Sobota

    Journal of Cell Science
    |November 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Increasing signal-to-noise ratio in over-determined Mueller matrices.

    Optics express·2023
    Same author

    Optimizing Mueller polarimetry in noisy systems through over-determination.

    Applied optics·2021
    Same author

    Effect of blend moisture and extrusion temperature on physical properties of everlasting pea-wheat extrudates.

    Journal of food science and technology·2015
    Same author

    Safety of intravenous thrombolysis for acute ischaemic stroke including concomitant neoplastic disease sufferers - experience from Poland.

    International journal of clinical practice·2015
    Same author

    The efficacy and safety of intravenous thrombolysis with alteplase in the treatment of ischaemic stroke in a rural hospital.

    Neurologia i neurochirurgia polska·2013
    Same author

    Note: Measuring breakdown characteristics during the hot re-ignition of high intensity discharge lamps using high frequency alternating current voltage.

    The Review of scientific instruments·2013

    Acanthamoeba cells form calcium-dependent electron-dense deposits (CaDD) on their plasma membrane. These deposits involve calcium and phosphate compounds, likely binding to specific membrane regions.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Microbiology

    Background:

    • Acanthamoeba cells exhibit unique structures at their plasma membrane.
    • Calcium ions play a role in cellular processes and structural formations.

    Purpose of the Study:

    • To investigate the nature and formation of calcium-dependent electron-dense deposits (CaDD) in Acanthamoeba.
    • To identify the chemical compounds involved in CaDD formation.

    Main Methods:

    • Glutaraldehyde fixation of Acanthamoeba cells in the presence of calcium ions and ATP.
    • Chemical analysis of trichloroacetic acid extracts from CaDD-containing cells.
    • Treatment with exogenous phosphatase and restoration experiments with pyrophosphate or orthophosphate.

    Main Results:

    Related Experiment Videos

    • Calcium-dependent electron-dense deposits (CaDD) were observed at the cytoplasmic surface of Acanthamoeba plasma membranes.
    • ATP stimulated CaDD formation.
    • Adenosine monophosphate, pyrophosphate, and inorganic phosphate were identified as probable compounds involved in CaDD.
    • Phosphatase treatment removed CaDD, revealing a fibrillar network.
    • CaDD could be restored using calcium ions with pyrophosphate or orthophosphate.

    Conclusions:

    • CaDD formation in Acanthamoeba is dependent on calcium ions and involves phosphate compounds.
    • Specialized subplasmalemmal calcium-binding microregions likely facilitate the deposition of calcium/phosphate salts.