Related Experiment Video
Updated: Jul 3, 2025
08:06
Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry
Published on: February 27, 2019
11.4K
YTHDF1's Regulatory Involvement in Breast Cancer Prognosis, Immunity, and the ceRNA Network
Wenting Luo1,2, Youjia Zhou1, Jiayang Wang3
1Department of Pathophysiology, Medical College, Nanchang University, 461 Bayi Road, Nanchang 330006, China.
International Journal of Molecular Sciences
|February 10, 2024
Summary
YTHDF1 protein is crucial in breast cancer progression and the immune microenvironment. Its regulatory networks, including ceRNA, offer potential therapeutic targets and biomarkers for breast cancer immunotherapy.
Area of Science:
- Oncology
- Molecular Biology
- Immunology
Background:
- YTH N6-methyladenosine RNA binding protein 1 (YTHDF1) is an m6A reader implicated in breast cancer development and its immune microenvironment.
- Competing endogenous RNA (ceRNA) networks are gaining attention for their role in cancer regulation and potential in tumor gene therapy.
Purpose of the Study:
- To investigate the relationship between YTHDF1 expression, breast cancer clinicopathology, and patient survival.
- To explore YTHDF1's role in tumor mutational burden, microsatellite instability, and the tumor microenvironment.
- To elucidate the interplay between YTHDF1 and ceRNA networks in breast cancer.
Main Methods:
- Analysis of TCGA and PrognoScan datasets to correlate YTHDF1 expression with survival and clinicopathology.
- Western blot validation of YTHDF1 expression in breast cancer tissues.
- Exploration of YTHDF1's functional associations with tumor mutational burden, microsatellite instability, and immune microenvironment components.
- Construction and analysis of novel ceRNA networks involving YTHDF1 and miR-378g.
Main Results:
- YTHDF1 is significantly expressed in breast cancer tissues.
- YTHDF1 expression correlates with breast cancer progression and may influence the tumor immune microenvironment.
- Three novel ceRNA networks (DR4-AS1-miR-378g-YTHDF1, HCG9-miR-378g-YTHDF1, taurine upregulated 1-miR-378g-YTHDF1) were identified.
- miR-378g was found to inhibit YTHDF1 expression, and the miR-378g/YTHDF1 axis impacts MDA-MB-231 cell proliferation.
Conclusions:
- YTHDF1 is a key m6A regulatory protein in breast cancer with a significant role in the immune microenvironment.
- YTHDF1 may serve as a prognostic biomarker and a potential therapeutic target for breast cancer immunotherapy.
- The identified ceRNA networks, particularly involving miR-378g and YTHDF1, highlight a novel regulatory axis impacting breast cancer cell growth.
Related Concept Videos
01:16TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
02:17The Tumor Microenvironment
6.6K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.6K
02:23Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
02:38Mitogens and the Cell Cycle
6.5K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.5K
01:24Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K