Microfluidic Paper-Based Device Incorporated with Silica Nanoparticles for Iodide Quantification in Marine Source...

Area of Science:

• Analytical Chemistry
• Biochemistry
• Materials Science

Background:

• Iodine is crucial for thyroid hormone synthesis, making iodine intake monitoring essential for public health.
• Iodine deficiency can lead to various health issues, necessitating accessible detection methods.
• Seaweed and dietary supplements are common sources of iodine, requiring accurate quantification.

Purpose of the Study:

• To develop and validate a microfluidic paper-based analytical device (μPAD) for sensitive iodide quantification.
• To enable simple, low-cost, and user-friendly point-of-care analysis of iodine in food and supplements.
• To assess the efficacy of the developed μPAD for analyzing real-world samples like seaweed and dietary supplements.

Main Methods:

• A microfluidic paper-based analytical device (μPAD) was designed for iodide detection.
• Iodide quantification was achieved via the iodine-catalyzed oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide.
• Powder silica was incorporated to enhance and standardize the colorimetric product intensity.

Main Results:

• The developed μPAD demonstrated successful iodide quantification in the range of 10–100 µM.
• A low detection limit of 3 µM for iodide was achieved.
• The μPAD was effectively applied to quantify iodide in seaweed and various dietary supplement samples.

Conclusions:

• The μPAD offers a valuable, disposable, and cost-effective tool for point-of-care iodine analysis.
• The device is suitable for use by untrained personnel, facilitating home-based monitoring of iodine intake.
• This analytical method contributes to preventing iodine deficiency through accessible health assessment.