Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Crossing Over01:30

Crossing Over

4.4K
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
4.4K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
Homologous Recombination02:31

Homologous Recombination

50.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.5K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K
Gene Conversion02:08

Gene Conversion

9.8K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
9.8K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.2K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prophase roles of replication protein A in crossover formation and meiotic progression.

Journal of microbiology (Seoul, Korea)·2026
Same author

Amphiregulin drives EGFR-dependent genome stability in colorectal cancer and represents a targetable vulnerability.

Oncogene·2026
Same author

PRX5 as a critical driver of colorectal cancer stemness and tumorigenicity.

Redox report : communications in free radical research·2026
Same author

Faulted ZnS:Mn,Cu Nanorods Provide Nanoscale Mechanoluminescence Excitation in a Biologically Relevant Force Range.

ACS nano·2026
Same author

Crossover interference mediates multiscale patterning along meiotic chromosomes.

Nature communications·2025
Same author

Genome-based exploration of volatile flavor diversity from food yeast species.

FEMS yeast research·2025

Related Experiment Video

Updated: Jul 3, 2025

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

8.0K

RPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination.

Jeong H Joo1, Soogil Hong1, Mika T Higashide2

  • 1Department of Life Sciences, Chung-Ang University, Seoul 06974, South Korea.

Nucleic Acids Research
|February 10, 2024
PubMed
Summary

Meiotic recombination relies on Rad52 interacting with replication protein A (RPA) to repair double-strand breaks (DSBs). Without this joint activity, RPA forms spikes, indicating failed DNA repair during meiosis.

More Related Videos

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

1.8K
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.2K

Related Experiment Videos

Last Updated: Jul 3, 2025

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

8.0K
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

1.8K
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.2K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Meiotic recombination initiates with programmed double-strand breaks (DSBs).
  • DSB repair involves resection, strand invasion, and DNA synthesis.
  • Rad52 protein is crucial for strand annealing in DSB repair pathways.

Purpose of the Study:

  • To investigate the role of Rad52-RPA interaction in meiotic recombination.
  • To understand the cause of RPA spikes observed during meiotic prophase.

Main Methods:

  • Physical analysis of DNA recombination in Saccharomyces cerevisiae.
  • Cytological observation of RPA localization during meiotic prophase.

Main Results:

  • Meiotic recombination's second-end capture is dependent on direct Rad52-RPA interaction.
  • Absence of Rad52-RPA joint activity leads to prominent RPA spikes at crossover sites.
  • RPA spikes represent unrepaired DSB ends during meiotic prophase.

Conclusions:

  • Rad52-RPA interaction is essential for efficient meiotic DSB repair.
  • RPA spikes serve as indicators of failed homologous recombination.
  • Rad52-RPA functions in meiosis mirror their roles in mitotic DSB repair.