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Bacterial Enzyme Assay for Mucin Glycan Degradation
Toshihiko Katoh1, Hisashi Ashida2
1Graduate School of Biostudies, Kyoto University, Kyoto, Japan. tkatoh@lif.kyoto-u.ac.jp.
None:
Bacterial sialidase and sulfoglycosidase may act on the acidic modifications of mucin O-glycans, producing sialic acid and 6-sulfated N-acetylglucosamine, respectively. Assays for these enzymes, using mucin as a substrate, are enabled by the detection and/or quantification of the free monosaccharides that are released by these enzymes. This chapter describes enzyme reactions with mucin, detection by thin-layer chromatography of sialic acid, and quantification of 6-sulfated N-acetylglucosamine by liquid chromatography-tandem mass spectrometry.
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