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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

88.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Updated: Jul 3, 2025

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
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Application of next-generation sequencing to identify different pathogens.

Aljuboori M Nafea1,2, Yuer Wang1, Duanyang Wang1

  • 1College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.

Frontiers in Microbiology
|February 13, 2024
PubMed
Summary

Next-generation sequencing (NGS) offers a powerful, high-throughput method for rapid pathogen identification. This technology is crucial for epidemiological monitoring and managing infectious diseases, overcoming limitations of traditional methods.

Keywords:
Sangerbacteriafunginext generation sequencingpathogens

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Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Accurate pathogen detection is vital for public health, disease control, and epidemiological surveillance.
  • Conventional methods like culture and mass spectrometry are often slow and have limitations.
  • Next-generation sequencing (NGS) presents a significant advancement in pathogen identification.

Purpose of the Study:

  • To review the principles and applications of NGS in identifying diverse pathogens.
  • To analyze the current challenges and future prospects of using NGS for clinical pathogen diagnosis.
  • To provide a theoretical foundation for NGS in pathogen identification.

Main Methods:

  • Review of existing literature on NGS technology and its application in pathogen identification.
  • Detailed introduction to NGS methodologies and their capabilities.
  • Analysis of case studies and research employing NGS for bacteria, fungi, and viruses.

Main Results:

  • NGS enables high-throughput, comprehensive pathogen identification across various microbial types.
  • Demonstrates superior speed and sensitivity compared to traditional diagnostic techniques.
  • Highlights the potential of NGS in outbreak investigations and clinical diagnostics.

Conclusions:

  • NGS is a transformative technology for rapid and accurate pathogen identification.
  • Its application in clinical settings is expanding, offering improved disease management strategies.
  • Further research and development are needed to fully realize NGS's potential in infectious disease diagnostics.