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Multi-regulated GDP-l-galactose phosphorylase calls the tune in ascorbate biosynthesis.

Pierre Baldet1, Kentaro Mori1, Guillaume Decros2

  • 1Université de Bordeaux, INRAE, UMR1332 BFP, 33882 Villenave d'Ornon, France.

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|February 13, 2024
PubMed
Summary

GDP-l-galactose phosphorylase (GGP) is key to controlling ascorbate synthesis, a vital process for plant stress response. Multi-level regulation fine-tunes GGP, crucial for adapting crops to climate change.

Keywords:
Abiotic stressPAS/LOVascorbatelightmulti-regulated GDP-l-galactose phosphorylasephotoreceptortranscription factorsuORFvitamin C

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Area of Science:

  • Plant biochemistry
  • Molecular biology
  • Stress physiology

Background:

  • Ascorbate is crucial for plant responses to abiotic and biotic stresses, exacerbated by climate change.
  • GDP-l-galactose phosphorylase (GGP) is identified as the primary regulatory enzyme in ascorbate biosynthesis.
  • Ascorbate levels are dynamic, varying with species, development, and environmental conditions.

Purpose of the Study:

  • To elucidate the multi-level regulatory mechanisms controlling GDP-l-galactose phosphorylase (GGP) activity.
  • To understand the role of GGP in plant stress responses and its potential for crop adaptation.

Main Methods:

  • Analysis of gene expression and protein levels of GGP.
  • Investigation of translational repression via upstream open reading frame (uORF).
  • Study of GGP activity modulation by a PAS/LOV photoreceptor and blue light.

Main Results:

  • GGP expression is induced by environmental and developmental factors, but protein levels are low due to translational repression by a uORF.
  • GGP activity is inhibited by a PAS/LOV photoreceptor, with blue light counteracting this inhibition.
  • Fine-tuning of ascorbate synthesis via multi-level GGP regulation allows for effective stress response.

Conclusions:

  • GGP regulation is complex, involving transcriptional, translational, and post-translational control.
  • Understanding GGP regulation is essential for enhancing plant stress tolerance.
  • This knowledge can aid in developing crop varieties resilient to future environmental challenges.