[Development of selective method for mebeverine detection in blood].

Area of Science:

• Forensic Toxicology
• Analytical Chemistry
• Biochemistry

Context:

• Mebeverine is a common antispasmodic drug.
• Accurate detection of mebeverine is crucial for diagnosing drug intoxication.
• Existing methods may yield false results due to sample degradation.

Purpose:

• To develop a reliable method for processing biological fluid samples for mebeverine detection.
• To establish a protocol that minimizes false positive results in drug intoxication diagnostics.
• To optimize sample preparation for sensitive and accurate mebeverine analysis.

Summary:

• A selective sample processing method involving aqueous phase extraction (pH 2-4) and enzymatic hydrolysis (papain, hyaluronidase) was developed for mebeverine isolation from blood.
• Native mebeverine degrades into veratric acid and mebeverine alcohol at alkaline pH.
• High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is recommended over gas chromatography-mass spectrometry (GC-MS) to prevent mebeverine degradation in the chromatograph injector.

Impact:

• Enables more accurate diagnosis of mebeverine-related drug intoxication.
• Provides a validated method for forensic and clinical toxicology laboratories.
• Improves the reliability of analytical results for mebeverine in biological samples.