Three-Four Photon Transition Mn(II) Complex Monitoring Lysosome-Related ATP in Real Time via Fluorescence Lifetime...

Area of Science:

• Biochemistry
• Chemical Biology
• Molecular Imaging

Background:

• Lysosomal adenosine triphosphate (ATP) levels are crucial for cellular processes.
• Existing methods for monitoring lysosomal ATP face limitations in targeting and resolution.
• Developing specific probes for in situ ATP detection in lysosomes is essential.

Purpose of the Study:

• To develop a novel probe for sensitive and specific in situ monitoring of lysosomal ATP.
• To investigate the probe's properties, including its targeting and fluorescence enhancement capabilities.
• To demonstrate the probe's utility in visualizing lysosomal ATP dynamics.

Main Methods:

• Synthesis and characterization of a manganese(II) complex (FX2-MnCl2) with near-infrared nonlinear optical properties.
• Utilizing multiphoton fluorescence imaging (MP-FI) and multiphoton fluorescence lifetime imaging microscopy (MP-FLIM).
• Employing dual recognition sites (diethoxy and manganese ions) for ATP binding.

Main Results:

• The FX2-MnCl2 probe exhibits a fivefold fluorescence enhancement in the presence of ATP.
• The probe demonstrates specific targeting to lysosomes.
• Lysosome-specific ATP migration was successfully monitored under near-infrared laser excitation.
• The probe exhibits a slight fluorescence lifetime change upon ATP binding.

Conclusions:

• FX2-MnCl2 is a novel multiphoton fluorescent complex for lysosomal ATP detection.
• The probe offers enhanced sensitivity and specificity compared to existing methods.
• This technology holds potential as a biomarker for lysosomal ATP in in vitro diagnostics.