Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nuclear Export01:42

Nuclear Export

3.6K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
3.6K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

7.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.7K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

4.6K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.6K
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

3.2K
Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
3.2K
Regulated mRNA Transport02:22

Regulated mRNA Transport

6.3K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.3K
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

5.7K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic changes and relationships among AI Literacy, job crafting, and career growth in new nurses in the AI era: A multicenter three-wave longitudinal study.

Nurse education today·2026
Same author

Quantitative comparison of methodologies for translation site imaging in living cells.

RNA (New York, N.Y.)·2026
Same author

Elevated desmoglein-2 expression in multiple myeloma is a prognostic marker across genomic subtypes with impact on high-risk cytogenetics and a distinct gene expression profile.

British journal of haematology·2026
Same author

Integrative pan-cancer analysis identifies CCHCR1 as a prognostic biomarker and therapeutic target driving EMT in hepatocellular carcinoma via PI3K/AKT activation.

Genes & diseases·2026
Same author

CD47 stabilizes ROBO2 to regulate glioblastoma progression by preventing ITCH-mediated ubiquitination.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

The One Click Wonder: a retrained automated segmentation pipeline that enables quantitative and modular analysis of <i>C. elegans</i> embryos.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jul 3, 2025

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.2K

Nuclear export of circular RNA.

Linh H Ngo1, Andrew G Bert2, B Kate Dredge2,3,4

  • 1RNA Biology and Cancer Laboratory, Peter MacCallum Cancer Centre and Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia.

Nature
|February 14, 2024
PubMed
Summary
This summary is machine-generated.

Researchers discovered a novel nuclear export pathway for circular RNAs (circRNAs). This pathway utilizes exportin-2 and IGF2BP1, regulated by Ran-GTP, offering new insights into circRNA regulation.

More Related Videos

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.3K
In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.5K

Related Experiment Videos

Last Updated: Jul 3, 2025

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.2K
Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.3K
In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.5K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • RNA Biology

Background:

  • Circular RNAs (circRNAs) are formed by precursor mRNA back-splicing.
  • circRNAs play roles in normal and cancerous cells.
  • circRNAs are primarily cytoplasmic, necessitating nuclear export.

Purpose of the Study:

  • To identify the specific pathway for nuclear export of circular RNAs.
  • To elucidate the molecular mechanisms governing circRNA nuclear export.

Main Methods:

  • Investigated the role of Ran-GTP, exportin-2, and IGF2BP1 in circRNA export.
  • Manipulated the nuclear Ran-GTP gradient using CRM1 inhibition/depletion.
  • Performed knockout/depletion of exportin-2.
  • Analyzed nuclear circRNA-binding proteins and their interactions.

Main Results:

  • Identified a novel pathway requiring Ran-GTP, exportin-2, and IGF2BP1 for circRNA nuclear export.
  • Modulating the nuclear Ran-GTP gradient affected circRNA export rates.
  • Exportin-2 depletion specifically inhibited circRNA nuclear export.
  • Ran-GTP enhances the interaction between IGF2BP1 and circRNAs.

Conclusions:

  • A Ran-GTP-dependent pathway involving exportin-2 and IGF2BP1 facilitates circRNA nuclear export.
  • This mechanism is analogous to protein export, distinct from mRNA export.
  • Adaptor proteins like IGF2BP1 are crucial for recruiting export machinery for circRNAs.