Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Principles Of Column Chromatography01:13

Principles Of Column Chromatography

6.8K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
6.8K
Chromatography: Introduction01:10

Chromatography: Introduction

4.3K
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
4.3K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

397
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
397
Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

2.3K
Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
2.3K
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

248
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
248
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

2.0K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Eco-friendly isolation and purification of oleuropein via three-phase high-speed counter-current chromatography and preparative HPLC.

Journal of chromatography. A·2025
Same author

HSCCC-Tchebichef moment regression approach for enhanced quantification of oleuropein in olive leaf extracts.

Journal of chromatography. A·2025
Same author

Recent Advances in Screening and Separating Active Components From Natural Products Based on High-Speed Countercurrent Chromatography.

Journal of separation science·2024
Same author

Comparison of Different High-Speed Countercurrent Chromatography Injection Modes for the Separation of Glabridin.

Journal of separation science·2024
Same author

Prediction model for retention volumes of the three-phase solvent system in high-speed countercurrent chromatography.

Journal of separation science·2024
Same author

Separation of astragaloside IV from Astragalus membranaceus based on high-speed countercurrent chromatography in continuous injection mode.

Phytochemical analysis : PCA·2024

Related Experiment Video

Updated: Jul 3, 2025

Curtain Flow Column: Optimization of Efficiency and Sensitivity
06:44

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

6.5K

Recent trends in multidimensional countercurrent chromatography.

Jules Muhire1,2, Xiao Sun1,2, Fu-Xin Zhang1,2

  • 1CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory of Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, P. R. China.

Journal of Separation Science
|February 15, 2024
PubMed
Summary

Multidimensional countercurrent chromatography (MDCCC) enhances separation efficiency for complex natural product mixtures. This technique breaks down intricate samples, enabling faster and more effective resolution of numerous components.

Keywords:
applicationsmultidimensional countercurrent chromatographymultiple columns systemnatural productsreview

More Related Videos

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
08:01

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Published on: December 15, 2015

7.4K
Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.0K

Related Experiment Videos

Last Updated: Jul 3, 2025

Curtain Flow Column: Optimization of Efficiency and Sensitivity
06:44

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

6.5K
Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
08:01

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Published on: December 15, 2015

7.4K
Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.0K

Area of Science:

  • Chromatography
  • Natural Product Chemistry
  • Separation Science

Background:

  • Countercurrent chromatography (CCC) is a high-efficiency, high-capacity preparative separation technique.
  • Complex natural product mixtures contain hundreds to thousands of components, posing significant separation challenges.
  • Traditional single-dimension CCC is often insufficient for resolving exceedingly complex mixtures in a practical timeframe.

Purpose of the Study:

  • To review and explore the principles, systems, and applications of multidimensional CCC (MDCCC).
  • To highlight MDCCC's advantages and limitations in separating complex mixtures.
  • To assess the prospective capacity of MDCCC for resolving intricate samples.

Main Methods:

  • Review of existing literature and studies on MDCCC.
  • Exploration of various MDCCC configurations, including multiple column systems and multilayer coil J-type setups.
  • Discussion of integrated on-line monitoring systems within MDCCC workflows.

Main Results:

  • MDCCC enables the separation of complex mixtures by dividing the separation process into sequential dimensions.
  • Smaller fractions from an initial separation dimension are analyzed individually, facilitating resolution.
  • Various MDCCC separation features have been studied, demonstrating its potential for complex sample analysis.

Conclusions:

  • MDCCC offers a powerful approach to overcome the limitations of single-dimension CCC for complex mixtures.
  • The technique shows significant promise for the efficient and relatively rapid resolution of intricate natural product samples.
  • Further exploration of MDCCC aspects, including system design and applications, is warranted.