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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

Computational tools supporting known miRNA identification.

Van-Quy Do¹, Chuc Hoang-Thi¹, Thanh-Truong Pham¹

¹Faculty of Applied Sciences, International School, Vietnam National University, Hanoi, Vietnam; Center for Biomedicine and Community Health, International School, Vietnam National University, Hanoi, Vietnam.

Progress in Molecular Biology and Translational Science

|February 15, 2024

Summary

Researchers need fast tools for analyzing small RNA sequencing (sRNA-seq) data. sRNAtoolbox offers integrated solutions for microRNA (miRNA) expression profiling and downstream analysis, enhancing research efficiency.

Keywords:

Small RNA Web server microRNA sRNAtoolbox

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Published on: August 21, 2019

Area of Science:

Molecular Biology
Bioinformatics
Genomics

Background:

Small RNAs, including microRNAs (miRNAs), are crucial gene expression regulators in animals and plants.
miRNAs are implicated in host-microbe interactions and host innate immunity.
Short RNAs in physiological fluids present potential as non-invasive biomarkers.

Approach:

This study reviews available miRNA research tools to assess their utility.
The sRNAtoolbox was selected to facilitate integrated small RNA research.
Emphasis was placed on the usability and standalone/integrated functionality of the tools.

Key Points:

sRNAtoolbox provides integrated solutions for small RNA expression profiling.
Tools can be used independently or to deepen analysis of sRNAbench results.
Usability is a key consideration in the evaluation of these bioinformatics tools.

Conclusions:

Efficient and user-friendly tools are essential for the growing field of small RNA research.
sRNAtoolbox aims to simplify integrated small RNA data analysis.
The evaluation provides an overview of current miRNA research tools, focusing on sRNAtoolbox.