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Related Concept Videos

Cancer Prevention02:59

Cancer Prevention

Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...
Cancer Prevention02:59

Cancer Prevention

Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...
Herpes01:28

Herpes

Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...
Hepatitis01:25

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...
Genital Herpes01:23

Genital Herpes

Genital herpes is a sexually transmitted infection primarily caused by herpes simplex virus type 2 (HSV-2), though herpes simplex virus type 1 (HSV-1) is increasingly implicated in genital infections, particularly among younger populations. Transmission occurs mainly through sexual contact, with asymptomatic viral shedding serving as a major route of spread. This characteristic makes HSV-2 difficult to control at a population level, as individuals may unknowingly transmit the virus even in the...

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Related Experiment Video

Updated: Jun 21, 2026

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
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AmpFire HPV and ScreenFire RS HPV validation trial.

Jun Hou1,2,3, Jerome L Belinson4,5, Hui Du1,2,3

  • 1Department of Obstetrics and Gynecology, Peking University Shenzhen Hospital, Shenzhen, China.

American Journal of Clinical Pathology
|February 16, 2024
PubMed
Summary

New human papillomavirus (HPV) screening assays demonstrate high accuracy and reproducibility. These validated HPV tests are suitable for low-resource settings, improving cervical cancer screening accessibility.

Keywords:
AmpFire HPV assayScreenFire HPV assayhierarchical analysisintra- and interlaboratory reproducibilityisothermal amplification

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Area of Science:

  • Molecular diagnostics
  • Gynecologic oncology
  • Public health

Background:

  • Cervical cancer screening relies on accurate human papillomavirus (HPV) detection.
  • Existing HPV assays require validation for diverse clinical settings, especially low-resource environments.
  • Atila Biosystems developed new HPV screening assays, AmpFire and ScreenFire RS, for enhanced detection capabilities.

Purpose of the Study:

  • To validate the clinical performance and reproducibility of Atila Biosystems' AmpFire and ScreenFire RS HPV screening assays.
  • To assess the diagnostic accuracy of these assays against established methods using clinical endpoints.
  • To evaluate the suitability of these assays for implementation in resource-limited settings.

Main Methods:

  • Validation of 556 self-collected specimens from the Chinese Multi-Site Screening Trial against cobas 4800 and SeqHPV.
  • Inclusion of samples with confirmed high-risk HPV (hrHPV) positivity and cervical intraepithelial neoplasia (CIN2+) endpoints.
  • Simulation of the VALGENT framework for inter- and intralaboratory validation, including hierarchical evaluation of the ScreenFire RS assay.

Main Results:

  • Both AmpFire and ScreenFire assays detected 98.1% of cases with CIN2 or higher.
  • High intralaboratory (95.13%-96.03%) and interlaboratory (93.68%-94.04%) concordance for overall hrHPV detection.
  • Excellent genotype-specific concordance and clinical guidance evaluation for ScreenFire RS, with high κ values (0.86-0.94).

Conclusions:

  • The AmpFire and ScreenFire RS assays exhibit excellent clinical performance and reproducibility.
  • The simplicity and reliability of these HPV assays make them highly applicable for cervical cancer screening in low-resource settings.
  • These validated assays offer a promising tool for improving global HPV detection and management strategies.