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A microfluidic platform integrating functional vascularized organoids-on-chip.

Clément Quintard^1,2,3, Emily Tubbs¹, Gustav Jonsson^4,5,6

¹Univ. Grenoble Alpes, CEA, IRIG/BGE, BIOMICS, 38000, Grenoble, France.

Nature Communications

|February 16, 2024

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Summary

This summary is machine-generated.

Researchers developed a new microfluidic platform to vascularize organoids-on-chips, enhancing their growth and function. This breakthrough enables better 3D tissue culture and organoid perfusion for advanced tissue engineering applications.

Area of Science:

Tissue Engineering
Microfluidics
Stem Cell Biology

Background:

Vascularizing three-dimensional cell cultures like organoids is essential for long-term tissue engineering.
Existing microfluidic devices often fail to replicate in vivo flow complexity and require intricate setups.
Vascularizing organoids-on-chips remains a significant challenge in the field.

Purpose of the Study:

To develop and evaluate a novel microfluidic platform for establishing and monitoring endothelial networks around various 3D cell aggregates.
To assess the functional integration and perfusion capabilities of these engineered vascular networks.
To investigate the impact of on-chip vascularization on organoid growth, maturation, and function.

Main Methods:

Development of a microfluidic platform designed to support endothelial network formation.

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Co-culture of endothelial networks with mesenchymal spheroids, pancreatic islet spheroids, and stem cell-derived vascular organoids.

Long-term (up to 30 days) monitoring of network formation and perfusion within the microphysiological system.

Assessment of organoid growth, maturation, and function post-vascularization.

Main Results:

Successful establishment of endothelial networks around diverse spheroids and vascular organoids.
Demonstrated functional intravascular perfusion of the cultured 3D tissues.
Significant enhancement in organoid growth, maturation, and overall function due to the vascularization method.
The engineered networks formed functional connections with endothelium-rich spheroids and vascular organoids.

Conclusions:

The developed microfluidic platform provides a viable solution for vascularizing diverse biological 3D tissues on-chip.
This system effectively supports organoid perfusion, improving their viability and functionality.
The study advances the field of organoids-on-chips by offering a more physiologically relevant microenvironment for tissue engineering.