Single-cell transcriptomic Atlas of aging macaque ocular outflow tissues
Jian Wu1,2, Chaoye Wang3, Shuhui Sun4,5,6
1Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Sciences, Beijing 100730, China.
Aging trabecular meshwork (TM) cells show mitochondrial dysfunction. Silencing APOE gene improves TM cell migration and function, offering potential glaucoma treatment insights.
Area of Science:
- Ophthalmology
- Cell Biology
- Genetics
Background:
- Trabecular meshwork (TM) degradation is linked to age-related eye diseases like primary open-angle glaucoma.
- The molecular mechanisms of TM aging remain largely unknown.
Purpose of the Study:
- To investigate the molecular basis of TM aging using single-cell transcriptomics.
- To identify key genes and pathways involved in TM dysfunction during aging.
Main Methods:
- Established a dynamic single-cell transcriptomic landscape of aged macaque TM.
- Classified TM cells into subtypes and clusters for in-depth analysis.
- Investigated the role of APOE gene in TM aging and function.
Main Results:
- Identified mitochondrial dysfunction as a key feature of TM aging.
- Discovered APOE as a crucial differentially expressed gene in aging TM.
- Showed that silencing APOE enhances cell migration, reduces apoptosis, and improves aqueous humor outflow.
Conclusions:
- APOE gene plays a significant role in TM aging, affecting cell migration and extracellular matrix regulation.
- Targeting APOE may offer a novel therapeutic strategy for glaucoma by restoring TM function and maintaining intraocular pressure.
More Related Videos
12:48In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma
Published on: May 11, 2015
10:14Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
Published on: May 26, 2023
