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Updated: Jul 2, 2025

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
Targeted metabolomics in human and animal biofluids and tissues using liquid chromatography coupled with tandem mass
Jill A Willency1, Yanzhu Lin2, Valentina Pirro1
1Technologies and Operations Group, Eli Lilly and Company, Indianapolis, IN 46225, USA.
Abstract:
Here, we present a targeted polar metabolomics protocol for the analysis of biofluids and frozen tissue biopsies using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). We describe steps for sample pretreatment, liquid-liquid extraction, and isolation of polar metabolites. We then detail procedures for target LC-MS/MS analysis. In this protocol, we focus on the analysis of plasma and serum samples. We also provide brief instructions on how to process other biological matrices as supplemental information. For complete details on the use and execution of this protocol, please refer to Coskun et al. (2022).1.
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