Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

907
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
907
RNA Stability01:53

RNA Stability

33.5K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.5K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.0K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

9.3K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.3K
RNA Editing02:23

RNA Editing

9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell DNA methylation analysis uncovers epigenetic pathways in the transformation of MDS to AML.

Leukemia·2026
Same author

Epigenetic silencing and pharmacological inhibition of EIF5A2 foster venetoclax sensitivity in acute myeloid leukaemia.

British journal of haematology·2026
Same author

The multiomics blueprint of the individual with the most extreme lifespan.

Cell reports. Medicine·2025
Same author

METTL16-mediated inhibition of MXD4 promotes leukemia through activation of the MYC-MAX axis.

Oncogene·2025
Same author

CLL crosstalk with naïve T cells enhances the differentiation of IL-22-producing T cells and CLL -cell survival.

Leukemia·2024
Same author

A SIRT7-dependent acetylation switch regulates early B cell differentiation and lineage commitment through Pax5.

Nature immunology·2024

Related Experiment Video

Updated: Jul 2, 2025

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.4K

Single-cell analysis of the epitranscriptome: RNA modifications under the microscope.

Eva Crespo-García1, Alberto Bueno-Costa1, Manel Esteller1,2,3,4

  • 1Cancer Epigenetics Group, Josep Carreras Leukaemia Research Institute (IJC), Badalona, Spain.

RNA Biology
|February 18, 2024
PubMed
Summary

Researchers review new single-cell technologies for mapping RNA modifications, like N6-methyladenosine (m6A), and studying the epitranscriptome. These methods advance gene expression understanding but still face challenges for efficient use.

Keywords:
InosineRNA modificationsSingle-cellepitranscriptomem6A

More Related Videos

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.7K
Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.2K

Related Experiment Videos

Last Updated: Jul 2, 2025

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.4K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.7K
Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.2K

Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Covalent RNA modifications add complexity to gene expression, challenging traditional RNA biology concepts.
  • Over 170 distinct RNA modifications have been identified, but their precise molecular functions remain largely unknown.
  • Mapping RNA modifications at single-cell resolution was previously unachievable.

Purpose of the Study:

  • To review technological advancements in single-cell methodologies for assessing RNA modifications.
  • To highlight strategies for studying the 'epitranscriptome' using these new techniques.
  • To focus on the application of these methods to N6-methyladenosine (m6A) modifications.

Main Methods:

  • Review of recent technological developments in single-cell RNA modification detection.
  • Analysis of novel strategies for functional assessment of RNA modifications in individual cells.
  • Focus on methodologies applicable to N6-methyladenosine (m6A) mapping.

Main Results:

  • Emergence of single-cell technologies enables mapping of specific RNA modifications.
  • Development of novel strategies for studying the epitranscriptome at single-cell resolution.
  • Advances facilitate the assessment and functional testing of RNA modifications like m6A.

Conclusions:

  • Technological progress allows for unprecedented insights into the epitranscriptome.
  • Despite advancements, significant challenges remain in the efficient application of these single-cell techniques.
  • Further development is needed to fully overcome existing technical hurdles in RNA modification analysis.