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Monocyte distribution width (MDW): study of reference values in blood donors
Ana García-Álvarez1,2, Yolanda Posada Franco1, Mercedes Martinez-Novillo González1
1Department of Medical Laboratory, Laboratory Medicine Institute, Instituto de Investigación Sanitaria Hospital Clínico San Carlos (IdISSC), Hospital Clínico San Carlos, Madrid, Spain.
Clinical Chemistry and Laboratory Medicine
|February 18, 2024
Summary
Monocyte distribution width (MDW) establishes a reference interval in healthy blood donors. This new sepsis biomarker aids early sepsis identification in clinical settings.
Area of Science:
- Clinical Chemistry
- Hematology
- Biomarker Discovery
Background:
- Monocyte distribution width (MDW) is an emerging biomarker for early sepsis indication.
- MDW aids in identifying patients at risk of developing sepsis.
- Accurate reference intervals are crucial for clinical interpretation of MDW.
Purpose of the Study:
- To establish the Monocyte distribution width (MDW) reference interval (RI) in a healthy blood donor population.
- To utilize EDTA-K2 as an anticoagulant for MDW analysis.
- To provide a reference for laboratories serving similar populations.
Main Methods:
- Analysis of 274 healthy blood donor samples.
- MDW measurements performed within 2 hours post-extraction.
- Estimation of RI using non-parametric, robust, and Harrell-Davis bootstrap methods, adhering to CLSI EP28-A3c guidelines.
Main Results:
- Non-parametric method yielded an RI of 14.77-21.13.
- Robust method resulted in an RI of 15.64-19.05.
- Harrell-Davis bootstrap method provided an RI of 14.73-21.14.
Conclusions:
- The non-parametric method's RI is recommended for clinical applicability, aligning with CLSI standards.
- The established RI serves as a valuable reference for other laboratories.
- This study contributes to the reliable clinical use of MDW in sepsis detection.
