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Related Experiment Video

Updated: May 7, 2026

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

A Potentially Versatile Enzyme Sensor Platform: Enzyme-Loaded, Tagged, Porous Polymeric Nanocapsules.

Bradley P Hambly¹, Chandler Sears¹, Bradford D Pendley¹

¹Department of Biomedical Engineering, University of Memphis, Memphis, Tennessee 38152, United States.

|February 19, 2024

Summary

This summary is machine-generated.

This study developed porous nanocapsules to protect enzymes like glucose oxidase (GOx), preserving their activity for biosensing applications. These stable enzyme nanocapsules show promise for industrial and medical uses.

Keywords:

encapsulation of enzymes enzyme activity measurement glucose concentration imaging glucose measurement glucose oxidase pH sensitive fluorescent tag porous polymeric nanocapsules

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Area of Science:

Biotechnology
Materials Science
Enzyme Engineering

Background:

Enzymes are vital industrial catalysts but suffer from poor stability.
Existing enzyme immobilization methods are often enzyme- or application-specific.
Enhanced enzyme stability is crucial for broader industrial and medical applications.

Purpose of the Study:

To explore the use of porous, indicator-tagged polymeric nanocapsules for enzyme encapsulation and substrate measurement.
To synthesize and characterize glucose oxidase (GOx)-loaded, fluorophore-tagged nanocapsules.
To evaluate the stability and activity of encapsulated GOx.

Main Methods:

Enzyme-loaded, fluorophore-tagged nanocapsules synthesized using self-assembled surfactant vesicle templates.
Measurement of GOx-catalyzed reaction rates to confirm preserved biological activity.
Evaluation of encapsulated GOx stability in the presence of hydrolytic enzymes.

Main Results:

GOx activity decay was less than 10% during encapsulation and purification.
Up to 30% of encapsulated GOx activity was retained for 3-5 days in the presence of hydrolytic enzymes.
Proof-of-concept: Fluorophore-tagged, GOx-loaded nanocapsules determined glucose concentrations (18-162 mg/dL) and enabled glucose imaging.

Conclusions:

Porous nanocapsules effectively protect encapsulated enzymes, preserving their activity.
The developed nanocapsules offer enhanced enzyme stability for practical applications.
This approach provides a versatile platform for enzyme immobilization and biosensing.