Structural Characterization and Functional Studies of Exopolysaccharide by Native Lacticaseibacillus rhamnosus P14...

Exopolysaccharides (EPS) are natural polymers synthesized by several microorganisms, including lactic acid bacteria (LAB). They are characterized by a great structural diversity, which gives them interesting biological and pharmacological properties. This work investigates the physicochemical and biological characterization of a new exopolysaccharide (EPS) produced by a wild Lacticaseibacillus rhamnosus P14. The functional groups, chemical bonds, and thermal and morphological properties of the purified EPS-P14 were determined using Fourier Transform Infrared, Nuclear Magnetic Resonance, and X-ray diffraction spectroscopies, as well as Thermo-gravimetric analysis, Differential Scanning Calorimetry and Scanning Electron Microscopy. The functional properties, namely antioxidant and emulsifying activities, were also assessed. The physicochemical analysis revealed that EPS-P14 is a porous and thermally stable polysaccharide with a degradation temperature of 307 °C. NMR and FT-IR studies identified it as a homogeneous α-D-glucan with mainly α-(1 → 6) glycosidic linkage and some α-(1 → 3) branching. EPS-P14 was highly water-soluble and exhibited strong emulsifying and stabilizing properties in a concentration-dependent manner. Furthermore, EPS-P14 demonstrated significant DPPH scavenging and ferric-reducing capacities. These findings suggest that EPS-P14 is a bioactive polysaccharide with potential effects, which could be a promising natural candidate for prospective application.