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Updated: Jul 2, 2025

Facile Preparation of Internally Self-assembled Lipid Particles Stabilized by Carbon Nanotubes

Facile Preparation of Internally Self-assembled Lipid Particles Stabilized by Carbon Nanotubes

Published on: February 19, 2016

Chimeric nanobody-decorated liposomes by self-assembly.

Md Mofizur Rahman^1,2, Jing Wang^3,4, Guosheng Wang^1,5

¹The Pq Laboratory of BiomeDx/Rx, Department of Biomedical Engineering, Binghamton University, Binghamton, NY, USA.

Nature Nanotechnology

|February 20, 2024

Summary

This summary is machine-generated.

This study introduces a novel, chemical-free method for creating targeted immunoliposomes using self-assembling chimeric nanobodies. These targeted liposomes effectively deliver drugs to HER2-cancer cells, enhancing treatment efficacy and patient survival.

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Last Updated: Jul 2, 2025

Facile Preparation of Internally Self-assembled Lipid Particles Stabilized by Carbon Nanotubes

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Published on: February 19, 2016

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

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Published on: March 17, 2023

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Published on: May 1, 2020

Area of Science:

Nanomedicine
Biophysics
Cancer Therapeutics

Background:

Liposomes offer advantages as drug delivery vehicles, including payload protection and improved biodistribution.
Challenges in immunoliposome manufacturing include targeting moiety dysfunction and payload loss, hindering commercial adoption.

Purpose of the Study:

To develop a chemical modification-free, one-step method for producing functional immunoliposomes.
To utilize a chimeric nanobody (cNB) for self-assembly into liposome bilayers for targeted drug delivery.

Main Methods:

A chimeric nanobody (cNB) comprising an anti-HER2 nanobody, linker, and transmembrane domain was engineered.
Self-assembly of cNB into liposome bilayers was achieved under facile conditions.
Encapsulation efficiency and cNB membrane anchoring capacity were quantified.

Main Results:

Up to 64% of therapeutic compounds were encapsulated into 100-nm liposomes.
Approximately 2,500 cNBs were anchored per liposome without steric hindrance.
Drug-loaded immunoliposomes demonstrated a 10- to 20-fold increase in cytotoxicity against HER2-overexpressing cancer cells.

Conclusions:

The biophysical approach enables efficient, one-step production of immunoliposomes.
Targeted immunoliposomes significantly enhance anti-cancer efficacy in vitro and in vivo.
This method presents a promising strategy for advancing immunoliposome-based cancer therapies.