Antiphotoaging effects of solvent fractions isolated from Allomyrina dichotoma larvae extract
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Updated: Jul 2, 2025
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Published on: August 23, 2024
Kyong Kim1, Eun-Young Park2, Dong-Jae Baek2
1Department of Food and Nutrition, Eulji University, Seongnam, South Korea.
Abstract:
Skin aging is affected by a variety of factors, including ultraviolet rays, oxidative stress, medications, smoking, and genetics. Among them, photo-aging accounts for about 80% of skin aging. The present study was evaluated to verify the potential of Allomyrina dichotoma larvae, which has recently been attracting attention as an edible insect, as an anti-aging substance. UVB irradiation at 100 mJ/cm2 was sufficient to induce photo-aging of fibroblasts within 24 h, which was alleviated after treatment with 70% ethanol extract of Allomyrina dichotoma larvae extract (ADLE). To obtain an extract from ADLE, which has a relatively high content of polyphenol compounds containing physiological activity, fractional solvent extraction was carried out using organic solvents such as hexane, chloroform, ethyl acetate, and butanol. Additionally, ethyl acetate and butanol fractions contributed to the inhibition of UVB-induced ROS production, cell damage, and senescence of fibroblasts. It was also confirmed that the two fractions can regulate the expression of MMP-1 and AP-1. In particular, the ethyl acetate fraction showed an excellent effect in recovering collagen decomposed by UVB. Therefore, these results suggest that ADLE has potential as a natural insect-derived biomaterial to inhibit UVB-induced photo-aging.
Insights
This study explored the anti-aging potential of edible insect extracts. Allomyrina dichotoma larvae extract (ADLE) effectively protected skin cells from UVB damage, suggesting its use in skincare.
Area of Science:
- Dermatology
- Biomaterials Science
- Entomology
Background:
- Photo-aging, primarily caused by UV radiation, constitutes a significant portion of skin aging.
- Edible insects, like Allomyrina dichotoma larvae, are gaining attention for their potential bioactive compounds.
- Polyphenols, known for physiological activity, are abundant in Allomyrina dichotoma larvae extract (ADLE).
Purpose of the Study:
- To investigate the anti-aging properties of Allomyrina dichotoma larvae extract (ADLE).
- To evaluate ADLE's efficacy in mitigating UVB-induced photo-aging in fibroblasts.
- To identify specific fractions of ADLE with significant anti-aging effects.
Main Methods:
- Fibroblast cell cultures were subjected to UVB irradiation to induce photo-aging.
- Cells were treated with 70% ethanol extract of Allomyrina dichotoma larvae (ADLE) and its fractions.
- Fractional solvent extraction (hexane, chloroform, ethyl acetate, butanol) was employed to isolate active components.
- Reactive oxygen species (ROS) production, cell damage, senescence, and collagen degradation markers (MMP-1, AP-1) were analyzed.
Main Results:
- ADLE treatment alleviated UVB-induced photo-aging in fibroblasts.
- Ethyl acetate and butanol fractions of ADLE inhibited UVB-induced ROS production, cell damage, and senescence.
- These fractions also regulated the expression of MMP-1 and AP-1.
- The ethyl acetate fraction demonstrated significant efficacy in restoring collagen degraded by UVB.
Conclusions:
- Allomyrina dichotoma larvae extract (ADLE) shows promise as a natural anti-aging substance.
- Specific fractions, particularly ethyl acetate, possess potent protective effects against UVB-induced skin aging.
- ADLE represents a potential insect-derived biomaterial for developing novel anti-photo-aging cosmetic or therapeutic agents.
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