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Complete genome sequences of two Klebsiella pneumoniae phages from Dakar, Senegal

Issa Ndiaye1,2, Laurent Debarbieux3, Moussa Moise Diagne4

  • 1Pole de Microbiologie, Institut Pasteur de Dakar, Dakar, Senegal.

Microbiology Resource Announcements
|February 20, 2024
PubMed

Insights

Researchers isolated two Klebsiella pneumoniae bacteriophages from Senegalese sewage. These phages, vKpIN17 and vKpIN18, were classified into distinct genera and families, highlighting phage diversity.

Area of Science:

  • Microbiology and Virology
  • Bacteriophage research
  • Environmental microbiology

Background:

  • Klebsiella pneumoniae is an opportunistic pathogen.
  • Bacteriophages (phages) are viruses that infect bacteria and can be used for phage therapy.
  • Isolation of novel phages is crucial for understanding bacterial-phage interactions and developing new therapeutic strategies.

Purpose of the Study:

  • To isolate and characterize bacteriophages targeting Klebsiella pneumoniae from environmental sources.
  • To determine the taxonomic classification of the isolated phages.

Main Methods:

  • Isolation of bacteriophages from sewage water samples collected in Dakar, Senegal.
  • Phage purification and characterization.
  • Genomic analysis to determine phage classification.

Main Results:

  • Two Klebsiella pneumoniae phages, designated vKpIN17 and vKpIN18, were successfully isolated.
  • Phage vKpIN17 was classified within the Przondovirus genus, Autographiviridae family, possessing a double-stranded DNA genome.
  • Phage vKpIN18 was assigned to the Weberuvirus genus, Drexlerviridae family.

Conclusions:

  • The study successfully isolated and identified two distinct bacteriophages of Klebsiella pneumoniae.
  • The findings contribute to the growing knowledge of phage diversity and their potential applications in combating bacterial infections.
  • The classification of these phages provides valuable data for the Autographiviridae and Drexlerviridae families.
Keywords:
Healthcare associated infectionsKlebsiella pneumoniaebacteriophage therapybacteriophages

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